DNA Test

[TimL_168]

To be honest, I can't really understand how any of them can claim to read 100% genetically DS. There are so many lines and most of them are at least to some degree outcrossed. I'm curious what the SPECIFIC markers are that they look at, and how those markers compare to other breeds and also how they compare to the markers other testing companies use.

[centrop67]

Under the health category, Radar passed with flying colors.



Did you know that Von Willebrand Disease Type I is common for Dutch Shepherds? Radar is clear.



They also generate a report that can be shared with your vet...

[Tim91118]

Was he checked for just VWF ?
What other diseases was he checked for and why were they not listed ?

[centrop67]

They identified VWF as being common to the breed, so it is highlighted on the health summary page.

There were 181 Genetic checks. There's not an easy way to share them all, but I will.

BTW - Embark is running a $30 discount on the breed + health test until 2/17. The code is PUPPYLOVE.

[centrop67]

Radar tested CLEAR for all these conditions:

Clinical
MDR1 Drug Sensitivity (MDR1) (Chromosome 14)
Hematologic (Blood)
P2Y12 Receptor Platelet Disorder (P2RY12) (Chromosome 23)
Factor IX Deficiency, Hemophilia B (F9 Exon 7, Terrier Variant) (Chromosome X)
Factor IX Deficiency, Hemophilia B (F9 Exon 7, Rhodesian Ridgeback Variant) (Chromosome X)
Factor VII Deficiency (F7 Exon 5) (Chromosome 22)
Factor VIII Deficiency, Hemophilia A (F8 Exon 10, Boxer Variant) (Chromosome X)
Factor VIII Deficiency, Hemophilia A (F8 Exon 11, Shepherd Variant 1) (Chromosome X)
Factor VIII Deficiency, Hemophilia A (F8 Exon 1, Shepherd Variant 2) (Chromosome X)
Thrombopathia (RASGRP2 Exon 5, Basset Hound Variant) (Chromosome 18)
Thrombopathia (RASGRP2 Exon 8) (Chromosome 18)
Thrombopathia (RASGRP2 Exon 5, American Eskimo Dog Variant) (Chromosome 18)
Von Willebrand Disease Type III, Type III vWD (VWF Exon 4) (Chromosome 27)
Von Willebrand Disease Type I (VWF) (Chromosome 27)
Von Willebrand Disease Type II, Type II vWD (VWF) (Chromosome 27)
Canine Leukocyte Adhesion Deficiency Type III, CLAD3 (FERMT3) (Chromosome 18)
Congenital Macrothrombocytopenia (TUBB1 Exon 1, Cavalier King Charles Spaniel Variant) (Chromosome 24)
Canine Elliptocytosis (SPTB Exon 30) (Chromosome 8)
Glanzmann's Thrombasthenia Type I (ITGA2B Exon 12) (Chromosome 9)
May-Hegglin Anomaly (MYH9) (Chromosome 10)
Prekallikrein Deficiency (KLKB1 Exon 8) (Chromosome 16)
Pyruvate Kinase Deficiency (PKLR Exon 5) (Chromosome 7)
Pyruvate Kinase Deficiency (PKLR Exon 7 Labrador Variant) (Chromosome 7)
Pyruvate Kinase Deficiency (PKLR Exon 7 Pug Variant) (Chromosome 7)
Pyruvate Kinase Deficiency (PKLR Exon 7 Beagle Variant) (Chromosome 7)
Pyruvate Kinase Deficiency (PKLR Exon 10) (Chromosome 7)
Trapped Neutrophil Syndrome (VPS13B) (Chromosome 13)
Ligneous Membranitis, LM (PLG) (Chromosome 1)
Platelet factor X receptor deficiency, Scott Syndrome (TMEM16F) (Chromosome 27)
Methemoglobinemia CYB5R3 (Chromosome 10)
Endocrine (Hormones)
Congenital Hypothyroidism (TPO, Tenterfield Terrier Variant) (Chromosome 17)
Immunologic (Immune)
Complement 3 Deficiency, C3 Deficiency (C3) (Chromosome 20)
Severe Combined Immunodeficiency (PRKDC) (Chromosome 29)
Severe Combined Immunodeficiency (RAG1) (Chromosome 18)
X-linked Severe Combined Immunodeficiency (IL2RG Variant 1) (Chromosome X)
X-linked Severe Combined Immunodeficiency (IL2RG Variant 2) (Chromosome X)
Ophthalmologic (Eyes)
Progressive Retinal Atrophy, rcd1 (PDE6B Exon 21 Irish Setter Variant) (Chromosome 3)
Progressive Retinal Atrophy, rcd3 (PDE6A) (Chromosome 4)
Progressive Retinal Atrophy, CNGA (CNGA1 Exon 9) (Chromosome 13)
Progressive Retinal Atrophy, prcd (PRCD Exon 1) (Chromosome 9)
Progressive Retinal Atrophy (CNGB1) (Chromosome 2)
Progressive Retinal Atrophy (SAG) (Chromosome 25)
Golden Retriever Progressive Retinal Atrophy 1, GR-PRA1 (SLC4A3) (Chromosome 37)
Golden Retriever Progressive Retinal Atrophy 2, GR-PRA2 (TTC8) (Chromosome 8)
Progressive Retinal Atrophy, crd1 (PDE6B) (Chromosome 3)
Progressive Retinal Atrophy, crd2 (IQCB1) (Chromosome 33)
Progressive Retinal Atrophy - crd4/cord1 (RPGRIP1) (Chromosome 15)
Collie Eye Anomaly, Choroidal Hypoplasia, CEA (NHEJ1) (Chromosome 37)
Achromatopsia (CNGA3 Exon 7 German Shepherd Variant) (Chromosome 10)
Achromatopsia (CNGA3 Exon 7 Labrador Retriever Variant) (Chromosome 10)
Autosomal Dominant Progressive Retinal Atrophy (RHO) (Chromosome 20)
Canine Multifocal Retinopathy (BEST1 Exon 2) (Chromosome 18)
Canine Multifocal Retinopathy (BEST1 Exon 5) (Chromosome 18)
Canine Multifocal Retinopathy (BEST1 Exon 10 Deletion) (Chromosome 18)
Canine Multifocal Retinopathy (BEST1 Exon 10 SNP) (Chromosome 18)
Glaucoma (ADAMTS10 Exon 9) (Chromosome 20)
Glaucoma (ADAMTS10 Exon 17) (Chromosome 20)
Glaucoma (ADAMTS17 Exon 11) (Chromosome 3)
Glaucoma (ADAMTS17 Exon 2) (Chromosome 3)
Hereditary Cataracts, Early-Onset Cataracts, Juvenile Cataracts (HSF4 Exon 9 Shepherd Variant) (Chromosome 5)
Primary Lens Luxation (ADAMTS17) (Chromosome 3)
Congenital Stationary Night Blindness (RPE65) (Chromosome 6)
Macular Corneal Dystrophy, MCD (CHST6) (Chromosome 5)
Urinary (Kidney and Bladder)
2,8-Dihydroxyadenine Urolithiasis, 2,8-DHA Urolithiasis (APRT) (Chromosome 5)
Cystinuria Type I-A (SLC3A1) (Chromosome 10)
Cystinuria Type II-A (SLC3A1) (Chromosome 10)
Cystinuria Type II-B (SLC7A9) (Chromosome 1)
Hyperuricosuria and Hyperuricemia or Urolithiasis, HUU (SLC2A9) (Chromosome 3)
Polycystic Kidney Disease, PKD (PKD1) (Chromosome 6)
Primary Hyperoxaluria (AGXT) (Chromosome 25)
Protein Losing Nephropathy, PLN (NPHS1) (Chromosome 1)
X-Linked Hereditary Nephropathy, XLHN (COL4A5 Exon 35, Samoyed Variant 2) (Chromosome X)
Autosomal Recessive Hereditary Nephropathy, Familial Nephropathy, ARHN (COL4A4 Exon 3) (Chromosome 25)
Multisystem
Primary Ciliary Dyskinesia, PCD (CCDC39 Exon 3) (Chromosome 34)
Congenital Keratoconjunctivitis Sicca and Ichthyosiform Dermatosis, Dry Eye Curly Coat Syndrome, CKCSID (FAM83H Exon 5) (Chromosome 13)
X-linked Ectodermal Dysplasia, Anhidrotic Ectodermal Dysplasia (EDA Intron 8) (Chromosome X)
Renal Cystadenocarcinoma and Nodular Dermatofibrosis, RCND (FLCN Exon 7) (Chromosome 5)
Canine Fucosidosis (FUCA1) (Chromosome 2)
Glycogen Storage Disease Type II, Pompe's Disease, GSD II (GAA) (Chromosome 9)
Glycogen Storage Disease Type IA, Von Gierke Disease, GSD IA (G6PC) (Chromosome 9)
Glycogen Storage Disease Type IIIA, GSD IIIA (AGL) (Chromosome 6)
Mucopolysaccharidosis Type I, MPS I (IDUA) (Chromosome 3)
Mucopolysaccharidosis Type IIIA, Sanfilippo Syndrome Type A, MPS IIIA (SGSH Exon 6 Variant 1) (Chromosome 9)
Mucopolysaccharidosis Type IIIA, Sanfilippo Syndrome Type A, MPS IIIA (SGSH Exon 6 Variant 2) (Chromosome 9)
Mucopolysaccharidosis Type VII, Sly Syndrome, MPS VII (GUSB Exon 5) (Chromosome 6)
Mucopolysaccharidosis Type VII, Sly Syndrome, MPS VII (GUSB Exon 3) (Chromosome 6)
Glycogen storage disease Type VII, Phosphofructokinase Deficiency, PFK Deficiency (PFKM Whippet and English Springer Spaniel Variant) (Chromosome 27)
Glycogen storage disease Type VII, Phosphofructokinase Deficiency, PFK Deficiency (PFKM Wachtelhund Variant) (Chromosome 27)
Lagotto Storage Disease (ATG4D) (Chromosome 20)
Neuronal Ceroid Lipofuscinosis 1, NCL 1 (PPT1 Exon 8) (Chromosome 15)
Neuronal Ceroid Lipofuscinosis 2, NCL 2 (TPP1 Exon 4) (Chromosome 21)
Neuronal Ceroid Lipofuscinosis 1, Cerebellar Ataxia, NCL4A (ARSG Exon 2) (Chromosome 9)
Neuronal Ceroid Lipofuscinosis 1, NCL 5 (CLN5 Border Collie Variant) (Chromosome 22)
Neuronal Ceroid Lipofuscinosis 6, NCL 6 (CLN6 Exon 7) (Chromosome 30)
Neuronal Ceroid Lipofuscinosis 8, NCL 8 (CLN8 English Setter Variant) (Chromosome 37)
Neuronal Ceroid Lipofuscinosis (MFSD8) (Chromosome 19)
Neuronal Ceroid Lipofuscinosis (CLN8 Australian Shepherd Variant) (Chromosome 37)
Neuronal Ceroid Lipofuscinosis 10, NCL 10 (CTSD Exon 5) (Chromosome 18)
Neuronal Ceroid Lipofuscinosis (CLN5 Golden Retriever Variant) (Chromosome 22)
Adult-Onset Neuronal Ceroid Lipofuscinosis (ATP13A2, Tibetan Terrier Variant) (Chromosome 2)
Late Onset Neuronal Ceroid Lipofuscinosis (ATP13A2, Australian Cattle Dog Variant) (Chromosome 2)
GM1 Gangliosidosis (GLB1 Exon 15 Shiba Inu Variant) (Chromosome 23)
GM1 Gangliosidosis (GLB1 Exon 15 Alaskan Husky Variant) (Chromosome 23)
GM1 Gangliosidosis (GLB1 Exon 2) (Chromosome 23)
GM2 Gangliosidosis (HEXB, Poodle Variant) (Chromosome 2)
GM2 Gangliosidosis (HEXA) (Chromosome 30)
Globoid Cell Leukodystrophy, Krabbe disease (GALC Exon 5) (Chromosome 8)
Other Systems
Autosomal Recessive Amelogenesis Imperfecta, Familial Enamel Hypoplasia (Italian Greyhound Variant) (Chromosome 13)
Autosomal Recessive Amelogenesis Imperfecta, Familial Enamel Hypoplasia (Parson Russell Terrier Variant) (Chromosome 13)
Persistent Mullerian Duct Syndrome, PMDS (AMHR2) (Chromosome 27)
Deafness and Vestibular Syndrome of Dobermans, DVDob, DINGS (MYO7A) (Chromosome 21)
Shar-Pei Autoinflammatory Disease, SPAID, Shar-Pei Fever (MTBP) (Chromosome 13)
Neurologic (Brain and Spinal Cord)
Alaskan Husky Encephalopathy, Subacute Necrotizing Encephalomyelopathy (SLC19A3) (Chromosome 25)
Alexander Disease (GFAP) (Chromosome 9)
Cerebellar Abiotrophy, Neonatal Cerebellar Cortical Degeneration, NCCD (SPTBN2) (Chromosome 18)
Cerebellar Ataxia, Progressive Early-Onset Cerebellar Ataxia (SEL1L) (Chromosome 8)
Cerebellar Hypoplasia (VLDLR) (Chromosome 1)
Spinocerebellar Ataxia, Late-Onset Ataxia, LoSCA (CAPN1) (Chromosome 18)
Spinocerebellar Ataxia with Myokymia and/or Seizures (KCNJ10) (Chromosome 38)
Benign Familial Juvenile Epilepsy, Remitting Focal Epilepsy (LGI2) (Chromosome 3)
Degenerative Myelopathy, DM (SOD1A) (Chromosome 31)
Fetal-Onset Neonatal Neuroaxonal Dystrophy (MFN2) (Chromosome 2)
Hypomyelination and Tremors (FNIP2) (Chromosome 15)
Shaking Puppy Syndrome, X-linked Generalized Tremor Syndrome (PLP) (Chromosome X)
Neuroaxonal Dystrophy, NAD (Spanish Water Dog Variant) (Chromosome 8)
Neuroaxonal Dystrophy, NAD (Rottweiler Variant) (Chromosome 5)
L-2-Hydroxyglutaricaciduria, L2HGA (L2HGDH) (Chromosome 0)
Neonatal Encephalopathy with Seizures, NEWS (ATF2) (Chromosome 36)
Polyneuropathy, NDRG1 Greyhound Variant (NDRG1 Exon 15) (Chromosome 13)
Polyneuropathy, NDRG1 Malamute Variant (NDRG1 Exon 4) (Chromosome 13)
Narcolepsy (HCRTR2 Intron 6) (Chromosome 12)
Progressive Neuronal Abiotrophy, Canine Multiple System Degeneration, CMSD (SERAC1 Exon 15) (Chromosome 1)
Progressive Neuronal Abiotrophy, Canine Multiple System Degeneration, CMSD (SERAC1 Exon 4) (Chromosome 1)
Juvenile Laryngeal Paralysis and Polyneuropathy, Polyneuropathy with Ocular Abnormalities and Neuronal Vacuolation, POANV (RAB3GAP1, Rottweiler Variant) (Chromosome 19)
Hereditary Sensory Autonomic Neuropathy, Acral Mutilation Syndrome, AMS (GDNF-AS) (Chromosome 4)
Juvenile-Onset Polyneuropathy, Leonberger Polyneuropathy 1, LPN1 (LPN1, ARHGEF10) (Chromosome 16)
Spongy Degeneration with Cerebellar Ataxia 1, SDCA1, SeSAME/EAST Syndrome (KCNJ10) (Chromosome 38)
Spongy Degeneration with Cerebellar Ataxia 2, SDCA2 (ATP1B2) (Chromosome 5)
Cardiac (Heart)
Dilated Cardiomyopathy, DCM1 (PDK4) (Chromosome 14)
Dilated Cardiomyopathy, DCM2 (TTN) (Chromosome 36)
Long QT Syndrome (KCNQ1) (Chromosome 18)
Muscular
Muscular Dystrophy (DMD, Cavalier King Charles Spaniel Variant 1) (Chromosome X)
Muscular Dystrophy (DMD Pembroke Welsh Corgi Variant ) (Chromosome X)
Muscular Dystrophy (DMD Golden Retriever Variant) (Chromosome X)
Centronuclear Myopathy (PTPLA) (Chromosome 2)
Exercise-Induced Collapse (DNM1) (Chromosome 9)
Inherited Myopathy of Great Danes (BIN1) (Chromosome 19)
Myostatin Deficiency, Bully Whippet Syndrome (MSTN) (Chromosome 37)
Myotonia Congenita (CLCN1 Exon 7) (Chromosome 16)
Myotonia Congenita (CLCN1 Exon 23) (Chromosome 16)
Myotubular Myopathy 1, X-linked Myotubular Myopathy, XL-MTM (MTM1, Labrador Variant) (Chromosome X)
Metabolic
Hypocatalasia, Acatalasemia (CAT) (Chromosome 18)
Pyruvate Dehydrogenase Deficiency (PDP1) (Chromosome 29)
Malignant Hyperthermia (RYR1) (Chromosome 1)
Gastrointestinal
Imerslund-Grasbeck Syndrome, Selective Cobalamin Malabsorption (CUBN Exon 53) (Chromosome 2)
Imerslund-Grasbeck Syndrome, Selective Cobalamin Malabsorption (CUBN Exon 8) (Chromosome 2)
Lundehund Syndrome (LEPREL1) (Chromosome 34)
Neuromuscular
Congenital Myasthenic Syndrome (CHAT) (Chromosome 28)
Congenital Myasthenic Syndrome (COLQ) (Chromosome 23)
Episodic Falling Syndrome (BCAN) (Chromosome 7)
Integument (Skin & Connective Tissues)
Dystrophic Epidermolysis Bullosa (COL7A1) (Chromosome 20)
Ectodermal Dysplasia, Skin Fragility Syndrome (PKP1) (Chromosome 7)
Ichthyosis, Epidermolytic Hyperkeratosis (KRT10) (Chromosome 9)
Ichthyosis (PNPLA1) (Chromosome 12)
Ichthyosis (SLC27A4) (Chromosome 9)
Ichthyosis (NIPAL4) (Chromosome 4)
Focal Non-Epidermolytic Palmoplantar Keratoderma, Pachyonychia Congenita (KRT16) (Chromosome 9)
Hereditary Footpad Hyperkeratosis (FAM83G) (Chromosome 5)
Hereditary Nasal Parakeratosis (SUV39H2) (Chromosome 2)
Musladin-Lueke Syndrome (ADAMTSL2) (Chromosome 9)
Bald Thigh Syndrome (IGFBP5) (Chromosome 37)
Skeletal
Cleft Lip and/or Cleft Palate (ADAMTS20) (Chromosome 27)
Hereditary Vitamin D-Resistant Rickets (VDR) (Chromosome 27)
Oculoskeletal Dysplasia 1, Dwarfism-Retinal Dysplasia, OSD1 (COL9A3, Labrador Retriever) (Chromosome 24)
Osteogenesis Imperfecta, Brittle Bone Disease (COL1A2) (Chromosome 14)
Osteogenesis Imperfecta, Brittle Bone Disease (SERPINH1) (Chromosome 21)
Osteogenesis Imperfecta, Brittle Bone Disease (COL1A1) (Chromosome 9)
Osteochondrodysplasia, Skeletal Dwarfism (SLC13A1) (Chromosome 14)
Skeletal Dysplasia 2, SD2 (COL11A2) (Chromosome 12)
Craniomandibular Osteopathy, CMO (SLC37A2) (Chromosome 5)
Chondrodystrophy and Intervertebral Disc Disease, CDDY/IVDD, Type I IVDD (FGF4 retrogene - CFA12) (Chromosome 12)
Chondrodystrophy, Norwegian Elkhound and Karelian Bear Dog Variant (ITGA10) (Chromosome 17)

[ladyjubilee]

Does Radar have any relatives?

A rescue here had a DS mix with the same genetic defect as Bramble. I always wondered if they were related.

[centrop67]

Radar has ~30 relatives in the Embark Database as of today.

Here's the top two by percentage:

[Tim91118]

Is that a picture of Jesse and Cleo ? If so Cleo is a Malinois ?

[Tim91118]

I scanned the list and did not see CHD ?
Canine Hip Dysplasia ?

[centrop67]

Cleo is a Malinois ?

Cleo looks like a Mal to me, but as you can see, Embark says he's a Dutch Shepherd. The list of relatives is mostly DS, but there are some identified as "Mixed Breed (%highpercentbreed)".

Embark provides a "chat' link to communicate with the relatives.

BTW - Embark is running a $30 discount on the breed + health test until 2/17. The code is PUPPYLOVE.

[centrop67]

I scanned the list and did not see CHD ?
Canine Hip Dysplasia ?

I don't see it either (???).

I can't find any specific mention that they do on embarkvet.com, but barkpost.com says that they (Embark does) test for it.

The only thing I found on embarkvet.com is an article that talks about the challenge to find all the chromosome combinations that contribute to diseases like hip dysplasia.

[ladyjubilee]

So which do you think is more accurate?


I'm very tempted and it is payday

[Tim91118]

I don’t know how a dog can be 100% Dutch Shepherd and have mixed breed relatives ?

[centrop67]

I don’t know how a dog can be 100% Dutch Shepherd and have mixed breed relatives ?

For parental relatives, I agree. For siblings and cousins, it's entirely possible to have mixed relatives. Embark doesn't provide any distinction except the % match of the DNA.

So which do you think is more accurate?

I wouldn't want to make that call. I do see that Embark has more features which is the biggest selling point to me.

[centrop67]

Embark just a added a new relative to Radar that matches 36% of his DNA

image.png (61.58 KiB) Viewed 8695 times

[Tim91118]

Check a couple of these dogs in the pedigree data base and you could get full blown pedigrees

[centrop67]

[ladyjubilee]

So since he is brindle with that much Malinois he is officially a Dutch Shepherd?

[centrop67]

Wow that's amazing. Idna's a rescue too, right? So you don't know anything about the parents.

[centrop67]

I just got the email notification of the new relative:

image.png (22.29 KiB) Viewed 8106 times